Part:BBa_K3098022:Design

Promoter(regulated by cI and lacI)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In order to better stop the RNA polymerase when it slide along the DNA, we apply two lacO sequences to our new promoter.

Source

cI regulated promoter is from lambda bacteriophage and has already been described in the part BBa_R0051. lacI binding site is from lac operator in the pET-28a